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I. Introduction

Welcome to the miRNA-mRNA expression atlas: a freely available knowledge base for studying the expression of miRNAs and their target genes in colorectal cancer.

Here is a step-by-step tutorial about the miRNA-mRNA expression atlas. Users can study the expression of miRNA and mRNA in colorectal cancer samples through web forms in many ways. Currently, users can search according to the names of miRNA (II.) and/or gene name (III.). miRNA-mRNA expression atlas can be used as a starting point in testing more targeted hypotheses and designing experiments using optimal miRNA and/or gene for further studies.

II. Search by miRNA

Insert a miRNA name ...

... or start typing and then select miRNA name from list of values to find more about miRNA and their target gene expression in colorectal cancer:

Report will open in new tab. It contains:

• 1. summary,
• 2. top 10 the most upregulated/downregulated target genes (according to selected miRNA) in colorectal cancer samples,
• 3. distribution of miRNA expression in colorectal cancer tissue samples,
• 4. list of target genes for selected miRNA

4. Catalog of differential expression of target gene and its miRNA and their correlation coefficient

Click on row and submit a selection with "Show graph" button

* form can also be used to search expression patterns of optional miRNA and gene (with unknown miRNA - target interaction)

Report will open in new tab:

Report contains:

• 1. summary,
• 2. distribution of miRNA/mRNA expression in colorectal cancer samples,
• 3. a table of differential expression of selected miRNA and gene in each colorectal cancer tissue sample

II. Search by gene

Insert a gene symbol ...

... or start typing and then select gene name from list of values to find more about this gene expression in colorectal cancer:

Report will open in new tab. It contains:

• 1. summary,
• 2. top 10 the most upregulated/downregulated miRNAS (according to selected gene) in colorectal cancer samples,
• 3. distribution of mRNA expression in colorectal cancer samples,
• 4. list of miRNAs for selected target gene

4. Catalog of differential expression for miRNA and target gene with correlation coefficient

Click on row and submit a selection with "Show graph" button

* form can be also used to search expression patterns of optional miRNA and gene (with unknown miRNA - target interaction)

Report will open in new tab:

Report contains:

• 1. summary,
• 2. distribution of miRNA/mRNA expression in colorectal cancer samples,
• 3. A table of differential expression of selected miRNA and gene in each colorectal cancer tissue sample

IV. Data

Sum up:

• Structure of used data
• Databases and tools used for miRNA-mRNA expression atlas development